Abstract

Background: More and more research is being done nowadays in an effort to develop plant-based anti-diabetic medications that are both safe and effective. Thus this study aimed to validate Invivo antidiabetic, antioxidant activity effect of leaves of Podocarpus gracilis, Hypoestes forskaolii, and Discopodium penninervum which is has been used in Ethiopian traditional health system in Mice. Method: The crude extract from the leaves of the three plants were tested for its anti-diabetic effects using a model of oral glucose-loaded (OGTT), normoglycemic, and single-dose-treated diabetic mice after extraction with 80% hydro methanol were done. At a doses of 100, 200, and 400 mg/kg, the extracts were examined and the plant leaf extract's antioxidant activity was evaluated using the DPPH assay. Result: The administration of D. penninervum leaf extract at all three dose (100, 200, and 400 mg/kg) has no significant blood glucose level reduction in Normoglycemic and OGTT model compared to control group. In contrast, it has significant BGL reduction with in single-dose-treated diabetic mice compare to control group. The administration of P. gracilis and H. forskaolii leaf extract orally at doses of 100, 200 and 400 mg/kg significantly decreased the blood glucose levels compared to the control diabetic mice in STZ diabetic model. The highest growth BGL reduction was recorded by extract of P. gracilis (45%) followed by H. forskaolii (44.1%) at 400 mg/kg of tested doses in single dose.  In the test for antioxidant activity, the highest IC50 values was observed for D. penninervum leaf extract and standard drug (ascorbic acid) were 10.23 μg/ml and 3.3μg/ml, respectively Conclusion: The present study, therefore, suggests that crude 80% hydro methanolic extracts of P. gracilis, H. forskaolii, and D. penninervum in Mice are safe and rich with active secondary metabolites which have promising antidiabetic, and anti-oxidants effects. Keywords: Antidiabetic, anti-oxidant, traditional medicine, Podocarpus gracilis, Hypoestes forskaolii, and Discopodium penninervum